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Cellular Technology Ltd
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PeproTech
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AnaSpec
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AnaSpec
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Image Search Results
Journal: Cells
Article Title: Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay
doi: 10.3390/cells4010040
Figure Lengend Snippet: Percentage of live, dead, and apoptotic T cells, and T cell functionality after storing PBMC at 4 °C or 37 °C overnight. PBMC were stored at 4 °C or 37 °C overnight. ( A ) The numbers of live, dead, and apoptotic cells was determined after overnight storage. ( B ) PBMC stored overnight at 4 °C (solid bars) and 37 °C (open bars) were resuspended in fresh medium and tested in an IFN-γ ELISPOT assay with different antigens. CMV peptide pool, EBV peptide pool, and Flu peptide pool were used to activate CD8 cells and Mumps and Mosquito antigens to activate CD4 cells.
Article Snippet: The antigens CEF peptide pool (CTL-CEF-002), CMV pool (PA-CMV-001), Flu pool (PA-Flu-001), and
Techniques: Enzyme-linked Immunospot